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Home Antibody All anti-VPS28 antibodies

Anti-VPS28 TRUEMAB Antibody Clone 1A8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA505691
  • VPS28 mouse monoclonal antibody,clone 1A8
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC414122) , 20ug Explanation
100ul
30ul
379 In Stock
CF505691
  • Carrier-free (BSA/glycerol-free) VPS28 mouse monoclonal antibody,clone 1A8
100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(2)
IHC(1)
IF(1)
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Also for VPS28 (NM_016208)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human VPS28(NP_057292) produced in HEK293T cell.
Clone NameClone 1A8 IsotypeIgG1
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF Suggested Dilutions WB 1:200~2000, IHC 1:150, IF 1:100,
Predicted MW Explanation 25.2 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens vacuolar protein sorting 28 homolog (S. cerevisiae) (VPS28), transcript variant 1
Alternative NameMGC60323
Database LinkNP_057292
Entrez Gene 51160 Human
Entrez Gene 66914 Mouse
Entrez Gene 300052 Rat
Entrez Gene 702412 Monkey
Entrez Gene 607677 Dog
FunctionThis gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally. [provided by RefSeq, Jul 2008].
Related Pathway Endocytosis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VPS28 (RC200025, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VPS28.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-VPS28 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-VPS28 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505691)
IF Image
Anti-VPS28 mouse monoclonal antibody (TA505691) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VPS28(RC200025).

 

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