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Home Antibody All anti-VPS28 antibodies

Anti-VPS28 TRUEMAB Antibody Clone OTI1A8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA505691 VPS28 mouse monoclonal antibody, clone OTI1A8 (formerly 1A8) 100ul
30ul
$379 In Stock
LC414122 VPS28 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF505691 Carrier-free (BSA/glycerol-free) VPS28 mouse monoclonal antibody, clone OTI1A8 (formerly 1A8) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)
IHC(1)
IF(1)
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Also for VPS28 (NM_016208)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human VPS28(NP_057292) produced in HEK293T cell.
Clone NameClone OTI1A8 IsotypeIgG1
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF Suggested Dilutions WB 1:200~2000, IHC 1:150, IF 1:100,
Predicted MW Explanation 25.2 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens vacuolar protein sorting 28 homolog (S. cerevisiae) (VPS28), transcript variant 1
Alternative NameMGC60323
Database LinkNP_057292
Entrez Gene 51160 Human
Entrez Gene 66914 Mouse
Entrez Gene 300052 Rat
Entrez Gene 702412 Monkey
Entrez Gene 607677 Dog
FunctionThis gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally. [provided by RefSeq, Jul 2008].
Related Pathway Endocytosis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VPS28 (RC200025, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VPS28.
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-VPS28 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-VPS28 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505691)
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Anti-VPS28 mouse monoclonal antibody (TA505691) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VPS28(RC200025).
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