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Anti-OTUB1 TRUEMAB Antibody Clone 5F6
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for OTUB1 (NM_017670)
|Full length human recombinant rotein of human OTUB1(NP_060140) produced in HEK293T cell.|
|WB, IF, FC
|| WB 1:2000, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens OTU domain, ubiquitin aldehyde binding 1 (OTUB1), transcript variant 1|
|HSPC263; OTB1; OTU1|
|The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OTUB1 (RC210648, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OTUB1.
Anti-OTUB1 mouse monoclonal antibody (TA505198) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY OTUB1(RC210648).
HEK293T cells transfected with either RC210648 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-OTUB1 antibody(TA505198), and then analyzed by flow cytometry.