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Home Antibody All anti-CAST antibodies

Anti-CAST TRUEMAB Antibody Clone 1E7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA504351
  • CAST (Calpastatin) mouse monoclonal antibody, Clone 1E7
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400663) , 20ug Explanation
100ul 379 In Stock
CF504351
  • Carrier-free (BSA/glycerol-free) CAST mouse monoclonal antibody,clone 1E7
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(1)
IHC(5)
FC(1)
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Also for CAST (NM_001750)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human CAST(NP_001741) produced in HEK293T cell.
Clone NameClone 1E7 IsotypeIgG1
Species ReactivityHuman Concentration0.62 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:2000, IHC 1:150, FLOW 1:100,
Predicted MW Explanation 84.8 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens calpastatin (CAST), transcript variant 1
Alternative NameBS-17; MGC9402; MGC9402; OTTHUMP00000158519; OTTHUMP00000158520; calpain inhibitor; heart-type calpastatin; sperm BS-17 component; calpastatin
Database LinkNP_001741
Entrez Gene 831 Human
FunctionThe protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAST (RC211776, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAST.
IHC Image
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504351)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504351)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504351)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504351)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504351)
FC Image
HEK293T cells transfected with either RC211776 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CAST antibody(TA504351), and then analyzed by flow cytometry.

 

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