Anti-PPP1R15A Antibody, clone 2B11

Also for PPP1R15A (NM_014330)

Gene Name	Homo sapiens protein phosphatase 1, regulatory subunit 15A (PPP1R15A)
Synonyms:	GADD34
Immunogen	Full length human recombinant protein of human PPP1R15A(NP_055145) produced in HEK293T cell.
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 2B11	Isotype	IgG1
Species Reactivity	Human , Mouse	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IF, FC	Suggested Dilutions	WB 1:500~2000, IF 1:100, FLOW 1:100,
Background	This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008].
Related Pathway

* Shipping is in business days

* OriGene provides validated application data and protocol, with money back guarantee.

WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP1R15A (RC200581, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP1R15A.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPP1R15A monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

IF Image

Anti-PPP1R15A mouse monoclonal antibody (TA504309) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPP1R15A(RC200581).

FC Image

HEK293T cells transfected with either RC200581 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PPP1R15A antibody(TA504309), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Hela cells, using anti-PPP1R15A antibody(TA504309),(Red), compared to a nonspecific negative control antibody,(Blue).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-PPP1R15A antibody(TA504309),(Red), compared to a nonspecific negative control antibody,(Blue).

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

OriGene Antibodies in recent publications

Anti-PPP1R15A TrueMAB Antibody clone 2B11

TrueMAB™ Antibodies - Made against Authentic Protein Antigens