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Home Antibody All anti-AKT1 antibodies

Anti-AKT1 TRUEMAB Antibody Clone 1G5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA504238
  • AKT1 mouse monoclonal antibody,clone 1G5
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401580) , 20ug Explanation
100ul 325 In Stock
CF504238
  • Carrier-free (BSA/glycerol-free) AKT1 mouse monoclonal antibody,clone 1G5
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(2)
IHC(3)
IF(1)
FC(1)
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Also for AKT1 (NM_005163)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombiant protein of human AKT1(NP_005154) produced in HEK293T cell.
Clone NameClone 1G5 IsotypeIgG1
Species ReactivityHuman , Dog , Rat , Mouse Concentration0.43 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 55.5 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 1
Alternative NameAKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
Database LinkNP_005154
Entrez Gene 207 Human
Entrez Gene 11651 Mouse
Entrez Gene 24185 Rat
Entrez Gene 490878 Dog
FunctionThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].
Related PathwayProtein KinaseES Cell Differentiation/IPSDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayApoptosisVEGF signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT1 (RC220257, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AKT1 monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-AKT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504238)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-AKT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504238)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-AKT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504238)
IF Image
Anti-AKT1 mouse monoclonal antibody (TA504238) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT1(RC220257).
FC Image
HEK293T cells transfected with either RC220257 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AKT1 antibody(TA504238), and then analyzed by flow cytometry.

 

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