Anti-FBXO21 Antibody, clone 1E1

Also for FBXO21 (NM_033624)

OriGene Data

Immunogen	Full length human recombinant protein of human FBXO21(NP_296373) produced in HEK293T cell.
Clone Name	clone 1E1	Isotype	IgG2a
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens F-box protein 21 (FBXO21), transcript variant 1
Alternative Name	FBX21
Database Link	NP_296373
Function	This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq].
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FBXO21 (RC223095, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FBXO21.

IHC Image

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-FBXO21 mouse monoclonal antibody. (TA503940)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FBXO21 mouse monoclonal antibody. (TA503940)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FBXO21 mouse monoclonal antibody. (TA503940)

IHC Image

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-FBXO21 mouse monoclonal antibody. (TA503940)

IHC Image

Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-FBXO21 mouse monoclonal antibody. (TA503940)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FBXO21 mouse monoclonal antibody. (TA503940)

IF Image

Anti-FBXO21 mouse monoclonal antibody (TA503940) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FBXO21(RC223095).

FC Image

HEK293T cells transfected with either RC223095 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FBXO21 antibody(TA503940), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Hela cells, using anti-FBXO21 antibody(TA503940),(Red), compared to a nonspecific negative control antibody,(Blue).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-FBXO21 antibody(TA503940),(Red), compared to a nonspecific negative control antibody,(Blue).

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Anti-FBXO21 TrueMAB Antibody clone 1E1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data