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Home Antibody All anti-RPN1 antibodies

Anti-RPN1 TRUEMAB Antibody clone 2E3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA503666
  • RPN1 (Ribophorin I) mouse monoclonal antibody, clone 2E3
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC418997) , 20ug Explanation
100ul $325 In Stock
CF503666
  • Carrier-free (BSA/glycerol-free) RPN1 mouse monoclonal antibody,clone 2E3
100ug $450 3-4 weeks
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WB(2)
IHC(6)
FC(1)

OriGene Data

ImmunogenFull length human recombinant protein of human RPN1(NP_002941) produced in HEK293T cell.
Clone Nameclone 2E3 IsotypeIgG1
Species ReactivityHuman , Dog Concentration0.45 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, FLOW 1:100,
Predicted MW Explanation 66.2 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens ribophorin I (RPN1)
Alternative NameOST1; RBPH1
Database LinkNP_002941
Entrez Gene 6184 Human
Entrez Gene 476516 Dog
FunctionThis gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq].
Related PathwayTransmembrane N-Glycan biosynthesisMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPN1 (RC201554, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPN1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RPN1 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503666)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503666)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503666)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-RPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503666)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503666)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503666)
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HEK293T cells transfected with either RC201554 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RPN1 antibody(TA503666), and then analyzed by flow cytometry.
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