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Anti-DAND5 TRUEMAB Antibody Clone OTI2H3
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for DAND5 (NM_152654)
|Human recombinant protein fragment corresponding to amino acids 23-189 of human DAND5(NP_689867) produced in E.coli.|
|WB, IHC, FC
||WB 1:500~2000, IHC 1:150, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens DAN domain family member 5, BMP antagonist (DAND5)|
|CER2; CERL2; CKTSF1B3; COCO; CRL2; DANTE; GREM3; SP1|
Entrez Gene 199699 Human
|This gene encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to bind Nodal and to inhibit the Nodal signaling pathway which patterns left/right body asymmetry. [provided by RefSeq].|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAND5 (RC221703, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAND5.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-DAND5 monoclonal antibody. (1:200)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DAND5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503555)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DAND5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503555)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DAND5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503555)
HEK293T cells transfected with either RC221703 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DAND5 antibody(TA503555), and then analyzed by flow cytometry.