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Home Antibody All anti-PPARA antibodies

Anti-PPARA TRUEMAB Antibody Clone 2D10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA503529
  • PPARA (PPAR alpha) mouse monoclonal antibody, Clone 2D10
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401560) , 20ug Explanation
100ul 325 In Stock
CF503529
  • Carrier-free (BSA/glycerol-free) PPARA mouse monoclonal antibody,clone 2D10
100ug $450 3-4 weeks
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WB(1)
IF(1)
FC(1)

OriGene Data

ImmunogenHuman recombinant protein fragment correspongding to amino acids 145-420 of human PPARA(NP_005027) produced in E.coli.
Clone NameClone 2D10 IsotypeIgG1
Species ReactivityHuman Concentration0.48 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:200, IF 1:100, FLOW 1:100,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens peroxisome proliferator-activated receptor alpha (PPARA), transcript variant 5
Alternative NamehPPAR; NR1C1; PPAR; PPARalpha
Database LinkNP_005027
Entrez Gene 5465 Human
FunctionPeroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq].
Related PathwayNuclear Hormone ReceptorTranscription FactorsDruggable Genome PPAR signaling pathwayAdipocytokine signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPARA (RC216176, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPARA.
IF Image
Anti-PPARA mouse monoclonal antibody (TA503529) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPARA(RC216176).
FC Image
HEK293T cells transfected with either RC216176 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PPARA antibody(TA503529), and then analyzed by flow cytometry.

 

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