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Anti-PDE1B TRUEMAB Antibody Clone 6E4
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for PDE1B (NM_000924)
|Full length human recombinant protein of human PDE1B(NP_000915) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 1|
Entrez Gene 5153 Human
|Cyclic nucleotide phosphodiesterases (PDEs) catalyze hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been classified into several families based on their biochemical properties. Members of the PDE1 family, such as PDE1B, are calmodulin (see MIM 114180)-dependent PDEs (CaM-PDEs) that are stimulated by a calcium-calmodulin complex (Repaske et al., 1992 [PubMed 1326532]).[supplied by OMIM].|
|Druggable Genome Purine metabolismCalcium signaling pathwayProgesterone-mediated oocyte maturation|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE1B (RC206588, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE1B.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDE1B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PDE1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503319)
Anti-PDE1B mouse monoclonal antibody (TA503319) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE1B(RC206588).
HEK293T cells transfected with either RC206588 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDE1B antibody(TA503319), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-PDE1B antibody(TA503319),(Red), compared to a nonspecific negative control antibody,(Blue).