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Home Antibody All anti-SIRT5 antibodies

Anti-SIRT5 TRUEMAB Antibody clone 2F1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA503232
  • SIRT5 mouse monoclonal antibody,clone 2F1
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC415885) , 20ug Explanation
100ul 325 In Stock
CF503232
  • Carrier-free (BSA/glycerol-free) SIRT5 mouse monoclonal antibody,clone 2F1
100ug $450 3-4 weeks
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WB(2)
IHC(1)
IF(1)
FC(1)
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Also for SIRT5 (NM_012241)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human SIRT5(NP_036373) produced in HEK293 cell.
Clone Nameclone 2F1 IsotypeIgG1
Species ReactivityHuman, Rat Concentration0.24 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens sirtuin 5 (SIRT5), transcript variant 1
Alternative NameSIR2L5
Database LinkNP_036373
Entrez Gene 23408 Human
Entrez Gene 306840 Rat
FunctionThis gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq].
Related PathwayTranscription FactorsDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (RC200189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SIRT5 monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-SIRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503232)
IF Image
Anti-SIRT5 mouse monoclonal antibody (TA503232) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5(RC200189).
FC Image
HEK293T cells transfected with either RC200189 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SIRT5 antibody(TA503232), and then analyzed by flow cytometry.

 

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