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Home Antibody All anti-EIF4E antibodies

Anti-EIF4E TRUEMAB Antibody Clone OTI5D11

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA503042 EIF4E mouse monoclonal antibody, clone OTI5D11 (formerly 5D11) 100ul $325 In Stock
LC400723 EIF4E HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF503042 Carrier-free (BSA/glycerol-free) EIF4E mouse monoclonal antibody, clone OTI5D11 (formerly 5D11) 100ug $450 3-4 weeks
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WB(2)
IHC(3)
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Also for EIF4E (NM_001968)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human EIF4E(NP_001959) produced in HEK293T cell.
Clone NameClone OTI5D11 IsotypeIgG1
Species ReactivityHuman Concentration0.5 mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB 1:2000, IHC 1:150,
Predicted MW Explanation 24.9 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1
Alternative NameAUTS19; CBP; EIF4E1; EIF4EL1; EIF4F
Database LinkNP_001959
Entrez Gene 1977 Human
FunctionAll eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM].
Related Pathway mTOR signaling pathwayInsulin signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF4E (RC207333, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF4E.
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Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-EIF4E monoclonal antibody.(1:200)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-EIF4E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503042)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-EIF4E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503042)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-EIF4E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503042)
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