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Anti-TP53 TRUEMAB Antibody Clone OTI2H6

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA503034 TP53 mouse monoclonal antibody, clone OTI2H6 (formerly 2H6) 100ul $325 In Stock
LC400186 TP53 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF503034 Carrier-free (BSA/glycerol-free) TP53 mouse monoclonal antibody, clone OTI2H6 (formerly 2H6) 100ug $450 3-4 weeks
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WB(1)
IHC(7)
IF(1)

OriGene Data

ImmunogenFull length human recombinant protein of human TP53(NP_000537) produced in HEK293T cell.
Clone NameClone OTI2H6 IsotypeIgG1
Species ReactivityHuman Concentration0.72 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:500~2000, IHC 1:150, IF 1:100,
Predicted MW Explanation 43.5 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens tumor protein p53 (TP53), transcript variant 1
Alternative NameBCC7; LFS1; P53; TRP53
Database LinkNP_000537
Entrez Gene 7157 Human
FunctionThis gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq].
Related PathwayStem cell - PluripotencyTranscription FactorsDruggable Genome MAPK signaling pathwayCell cyclep53 signaling pathwayApoptosisWnt signaling pathwayNeurotrophin signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53 (RC200003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53.
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503034)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503034)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503034)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503034)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503034)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503034)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503034)
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Anti-TP53 mouse monoclonal antibody (TA503034) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53(RC200003).
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