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Anti-SNAP25 TRUEMAB Antibody Clone OTI4B2
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for SNAP25 (NM_003081)
|Full length human recombinant protein of human SNAP25(NP_003072) produced in HEK293 cell.|
|WB, IHC, IF, FC
||WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens synaptosomal-associated protein, 25kDa (SNAP25), transcript variant 1|
|bA416N4.2; dJ1068F16.2; RIC-4; RIC4; SEC9; SNAP; SNAP-25|
Entrez Gene 6616 Human
|Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq].|
|Druggable Genome SNARE interactions in vesicular transport|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAP25 (RC202068, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAP25.
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-SNAP25 monoclonal antibody.(1:200)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SNAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502968)
Anti-SNAP25 mouse monoclonal antibody (TA502968) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SNAP25(RC202068).
HEK293T cells transfected with either RC202068 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SNAP25 antibody(TA502968), and then analyzed by flow cytometry.