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Home Antibody All anti-PNMT antibodies

Anti-PNMT TRUEMAB Antibody Clone OTI2D5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502826 PNMT mouse monoclonal antibody, clone OTI2D5 (formerly 2D5) 100ul $325 In Stock
LC400946 PNMT HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502826 Carrier-free (BSA/glycerol-free) PNMT mouse monoclonal antibody, clone OTI2D5 (formerly 2D5) 100ug $450 3-4 weeks
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WB(1)
IHC(1)
IF(2)
FC(3)

OriGene Data

ImmunogenFull length human recombinant protein of human PNMT (NP_002677) produced in HEK293T cell.
Clone NameClone OTI2D5 IsotypeIgG2a
Species ReactivityHuman Concentration0.7 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
Predicted MW Explanation 30.7 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens phenylethanolamine N-methyltransferase (PNMT), transcript variant 1
Alternative NamePENT; PNMTase
Database LinkNP_002677
Entrez Gene 5409 Human
FunctionThe product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. [provided by RefSeq].
Related PathwayDruggable Genome Tyrosine metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNMT (RC206586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PNMT.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502826)
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Anti-PNMT mouse monoclonal antibody (TA502826) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PNMT(RC206586 ).
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Immunofluorescent staining of HeLa cells using anti-PNMT mouse monoclonal antibody (TA502826).
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HEK293T cells transfected with either RC206586 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PNMT antibody(TA502826), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Hela cells, using anti-PNMT antibody(TA502826),(Red), compared to a nonspecific negative control antibody,(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-PNMT antibody(TA502826),(Red), compared to a nonspecific negative control antibody,(Blue).
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