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Anti-PNMT TRUEMAB Antibody Clone OTI2D5
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for PNMT (NM_002686)
|Full length human recombinant protein of human PNMT (NP_002677) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens phenylethanolamine N-methyltransferase (PNMT), transcript variant 1|
Entrez Gene 5409 Human
|The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. [provided by RefSeq].|
|Druggable Genome Tyrosine metabolismMetabolic pathways|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNMT (RC206586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PNMT.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502826)
Anti-PNMT mouse monoclonal antibody (TA502826) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PNMT(RC206586 ).
Immunofluorescent staining of HeLa cells using anti-PNMT mouse monoclonal antibody (TA502826).
HEK293T cells transfected with either RC206586 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PNMT antibody(TA502826), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-PNMT antibody(TA502826),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-PNMT antibody(TA502826),(Red), compared to a nonspecific negative control antibody,(Blue).