Anti-CDK2 Antibody, clone 1C4

Also for CDK2 (NM_001798)

OriGene Data

Immunogen	Full length human recombinant protein of human CDK2 (NP_001789) produced in HEK293T cell.
Clone Name	clone 1C4	Isotype	IgG2a
Species Reactivity	Human, Monkey	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IF, FC	Suggested Dilutions	WB 1:500~2000, IF 1:100, FLOW 1:100
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens cyclin-dependent kinase 2 (CDK2), transcript variant 1
Alternative Name	p33(CDK2)
Database Link	NP_001789
Function	The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq].
Related Pathway	Cell cycle Delta-Notch Signaling Pathway TGF Beta Signaling Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDK2 (RC200494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDK2.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CDK2 monoclonal antibody.

IF Image

Anti-CDK2 mouse monoclonal antibody (TA502807) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDK2(RC200494).

IF Image

Immunofluorescent staining of HeLa cells using anti-CDK2 mouse monoclonal antibody (TA502807).

FC Image

HEK293T cells transfected with either RC200494 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CDK2 antibody(TA502807), and then analyzed by flow cytometry.

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Anti-CDK2 TrueMAB Antibody clone 1C4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data