Anti-ADH1B Antibody, clone 4F12

Also for ADH1B (NM_000668)

OriGene Data

Immunogen	Full length human recombinant protein of human ADH1B (NP_000659) produced in HEK293T cell.
Clone Name	clone 4F12	Isotype	IgG2a
Species Reactivity	Human , Dog , Rat	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:500, IHC 1:150, IF 1:100, FLOW 1:100
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B)
Alternative Name	ADH2
Database Link	NP_000659
Function	The protein encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. [provided by RefSeq].
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ADH1B (RC205391, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADH1B.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ADH1B monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ADH1B mouse monoclonal antibody. (TA502777)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ADH1B mouse monoclonal antibody. (TA502777)

IHC Image

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-ADH1B mouse monoclonal antibody. (TA502777)

IHC Image

Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-ADH1B mouse monoclonal antibody. (TA502777)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ADH1B mouse monoclonal antibody. (TA502777)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ADH1B mouse monoclonal antibody. (TA502777)

IHC Image

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-ADH1B mouse monoclonal antibody. (TA502777)

IHC Image

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ADH1B mouse monoclonal antibody. (TA502777)

IF Image

Anti-ADH1B mouse monoclonal antibody (TA502777) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ADH1B(RC205391 ).

IF Image

Immunofluorescent staining of HeLa cells using anti-ADH1B mouse monoclonal antibody (TA502777).

FC Image

HEK293T cells transfected with either RC205391 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ADH1B antibody(TA502777), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Hela cells, using anti-ADH1B antibody(TA502777),(Red), compared to a nonspecific negative control antibody,(Blue).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-ADH1B antibody(TA502777),(Red), compared to a nonspecific negative control antibody,(Blue).

Anti-ADH1B TrueMAB Antibody clone 4F12

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data