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Home Antibody All anti-CHCHD5 antibodies

Anti-CHCHD5 TRUEMAB Antibody Clone OTI9E11

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502668 CHCHD5 mouse monoclonal antibody, clone OTI9E11 (formerly 9E11) 100ul $379 In Stock
LC410209 CHCHD5 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502668 Carrier-free (BSA/glycerol-free) CHCHD5 mouse monoclonal antibody, clone OTI9E11 (formerly 9E11) 100ug $450 3-4 weeks
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WB(2)
IHC(1)
FC(3)
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Also for CHCHD5 (NM_032309)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human CHCHD5 (NP_115685) produced in E.coli.
Clone NameClone OTI9E11 IsotypeIgG1
Species ReactivityHuman Concentration0.44 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:2000, IHC 1:150, FLOW 1:100
Predicted MW Explanation 12.2 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens coiled-coil-helix-coiled-coil-helix domain containing 5 (CHCHD5), transcript variant 1
Alternative NameC2orf9; MIC14
Database LinkNP_115685
Entrez Gene 84269 Human
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHCHD5 (RC202606, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHCHD5.
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Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-CHCHD5 monoclonal antibody.(1:200)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CHCHD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502668)
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HEK293T cells transfected with either RC202606 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CHCHD5 antibody(TA502668), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Hela cells, using anti-CHCHD5 antibody(TA502668),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-CHCHD5 antibody(TA502668),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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