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Home Antibody All anti-ARFGAP1 antibodies

Anti-ARFGAP1 TRUEMAB Antibody Clone OTI1F9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502592 ARFGAP1 mouse monoclonal antibody, clone OTI1F9 (formerly 1F9) 100ul $325 In Stock
LC406240 ARFGAP1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502592 Carrier-free (BSA/glycerol-free) ARFGAP1 mouse monoclonal antibody, clone OTI1F9 (formerly 1F9) 100ug $450 3-4 weeks
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WB(2)
IF(1)
FC(3)
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Also for ARFGAP1 (NM_175609)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ARFGAP1 (NP_783202) produced in HEK293T cell.
Clone NameClone OTI1F9 IsotypeIgG2b
Species ReactivityHuman, Monkey Concentration0.75 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:500~2000, IF 1:100, FLOW 1:100
Predicted MW Explanation 45.5 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 2
Alternative NameARF1GAP; HRIHFB2281
Database LinkNP_783202
Entrez Gene 55738 Human
Entrez Gene 719734 Monkey
FunctionThe protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Related Pathway Endocytosis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARFGAP1 (RC206987, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARFGAP1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARFGAP1 monoclonal antibody.
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Anti-ARFGAP1 mouse monoclonal antibody (TA502592) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARFGAP1(RC206987).
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HEK293T cells transfected with either RC206987 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ARFGAP1 antibody(TA502592), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Hela cells, using anti-ARFGAP1 antibody(TA502592),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-ARFGAP1 antibody(TA502592),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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