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Home Antibody All anti-ATP6V1B1 antibodies

Anti-ATP6V1B1 TRUEMAB Antibody Clone OTI6D2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA502519 ATP6V1B1 mouse monoclonal antibody, clone OTI6D2 (formerly 6D2) 100ul 325
In Stock
LC400635 ATP6V1B1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502519 Carrier-free (BSA/glycerol-free) ATP6V1B1 mouse monoclonal antibody, clone OTI6D2 (formerly 6D2) 100ug $450 3-4 weeks
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Also for ATP6V1B1 (NM_001692)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ATP6V1B1(NP_001683) produced in HEK293T cell.
Clone NameClone OTI6D2 IsotypeIgG1
Species ReactivityHuman Concentration0.73 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:2000, IF 1:100, FLOW 1:100,
Predicted MW Explanation 56.7 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1 (ATP6V1B1)
Alternative NameATP6B1; RTA1B; VATB; VMA2; VPP3
Database LinkNP_001683
Entrez Gene 525 Human
FunctionThis gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain B subunit isoforms and is found in the kidney. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. [provided by RefSeq].
Related PathwayDruggable Genome Oxidative phosphorylationMetabolic pathwaysVibrio cholerae infectionEpithelial cell signaling in Helicobacter pylori infection

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1B1 (RC209462, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6V1B1.

WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATP6V1B1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

IF Image
Anti-ATP6V1B1 mouse monoclonal antibody (TA502519) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP6V1B1(RC209462).

FC Image
HEK293T cells transfected with either RC209462 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ATP6V1B1 antibody(TA502519), and then analyzed by flow cytometry.


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