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OriGene Antibodies in recent publications
A common polymorphism in the LDL receptor gene has multiple effects on LDL receptor function Hum. Mol. Genet., Apr 2013; 22: 1424 - 1431. [anti-DDK]
Excitotoxicity Upregulates SARM1 Protein Expression and Promotes Wallerian-Like Degeneration of Retinal Ganglion Cells and Their Axons Invest. Ophthalmol. Vis. Sci., Apr 2013; 54: 2771 - 2780. [SARM1]
Modulation of TET2 expression and 5-methylcytosine oxidation by the CXXC domain protein IDAX Nature 497, 122-126 doi:10.1038/nature12052 [anti-DDK]
Natriuretic Peptide Receptor-3 Gene (NPR3): Nonsynonymous Polymorphism Results in Significant Reduction in Protein Expression Because of Accelerated Degradation Circ Cardiovasc Genet, Apr 2013; 6: 201 - 210. [NPR3]
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 Home All anti-BECN1 antibodies

Anti-BECN1 TrueMAB Antibody clone 3H8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Related Products Conjugation/Bulk FAQs
SKU Description Amount Price Availability*  
TA502517
  • Purified BECN1 (Beclin 1) mouse monoclonal antibody, clone 3H8
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401239) , 20ug
 100ul $325 In Stock Add to Shopping Cart
CF502517
  • Carrier-free (BSA/glycerol-free) BECN1 mouse monoclonal antibody, clone 3H8
100ug $450 3-4 weeks Add to Shopping Cart
WB(2)
IHC(3)
IF(1)
FC(1)
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Also for BECN1 (NM_003766)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
Gene NameHomo sapiens beclin 1, autophagy related (BECN1)
Synonyms:ATG6; beclin1; VPS30
ImmunogenFull length human recombinant protein of human BECN1 (NP_003757) produced in HEK293T cell.
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Nameclone 3H8 IsotypeIgG2a
Species ReactivityHuman , Rat , Monkey Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *WB, IHC, IF, FC Suggested Dilutions WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
BackgroundBeclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM, Jul 2010].
Related Pathway
Senescence and Autophagy

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BECN1 (RC201629, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BECN1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BECN1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BECN1 mouse monoclonal antibody. (TA502517)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-BECN1 mouse monoclonal antibody. (TA502517)
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-BECN1 mouse monoclonal antibody. (TA502517)
IF Image
Anti-BECN1 mouse monoclonal antibody (TA502517) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BECN1(RC201629).
FC Image
HEK293T cells transfected with either RC201629 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BECN1 antibody(TA502517), and then analyzed by flow cytometry.

 

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