Anti-BTRC Antibody, clone 1F10

Also for BTRC (NM_033637)

OriGene Data

Immunogen	Human recombinant protein fragment corresponding to amino acids 52-354 of human BTRC(NP_378663) produced in HEK293T cell.
Clone Name	clone 1F10	Isotype	IgG1
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, FC	Suggested Dilutions	WB 1:2000, FLOW 1:100,
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens beta-transducin repeat containing E3 ubiquitin protein ligase (BTRC), transcript variant 1
Alternative Name	BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A; FBXW1; FBXW1A; FWD1
Database Link	NP_378663
Function	This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6. [provided by RefSeq, Mar
Related Pathway	Cell cycle Hedgehog signaling pathway TGF Beta Signaling Pathway Wnt Signaling Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTRC (RC207025, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTRC.

FC Image

HEK293T cells transfected with either RC207025 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BTRC antibody(TA502463), and then analyzed by flow cytometry.

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Anti-BTRC TrueMAB Antibody clone 1F10

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OriGene Data

Reference Data