Anti-PRKCE Antibody, clone 2E9

Also for PRKCE (NM_005400)

OriGene Data

Immunogen	Full length human recombinant protein of human PRKCE (NP_005391) produced in HEK293T cell.
Clone Name	clone 2E9	Isotype	IgG1
Species Reactivity	Human , Dog , Monkey	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IF, FC	Suggested Dilutions	WB 1:1000~2000, IF 1:100, FLOW 1:100,
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens protein kinase C, epsilon (PRKCE)
Alternative Name	nPKC-epsilon; PKCE
Database Link	NP_005391
Function	Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq].
Related Pathway	Wnt Signaling Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKCE (RC217702, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKCE.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PRKCE monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

IF Image

Anti-PRKCE mouse monoclonal antibody (TA502438) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKCE(RC217702).

FC Image

HEK293T cells transfected with either RC217702 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PRKCE antibody(TA502438), and then analyzed by flow cytometry.

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Anti-PRKCE TrueMAB Antibody clone 2E9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data