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Anti-BCHE TRUEMAB Antibody clone 1E1
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for BCHE (NM_000055)
|Full length human recombinant protein of human BCHE (NP_000046) produced in HEK293T cell. |
|Human , Dog
|| WB 1:500~2000, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens butyrylcholinesterase (BCHE)|
|CHE1; CHE2; E1|
|Mutant alleles at the BCHE locus are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage. [provided by RefSeq, Jul 2008]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCHE (RC202198, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCHE.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BCHE monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC202198 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BCHE antibody(TA502307), and then analyzed by flow cytometry.