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Home Antibody All anti-DNAJB1 antibodies
KO-Validated

Anti-DNAJB1 TRUEMAB Antibody Clone OTI1F9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (1) Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA502195 DNAJB1 (Hsp40) mouse monoclonal antibody, clone OTI1F9 (formerly 1F9) 100ul $325 In Stock
LC401851 DNAJB1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502195 Carrier-free (BSA/glycerol-free) DNAJB1 mouse monoclonal antibody, clone OTI1F9 (formerly 1F9) 100ug $450 4 Weeks
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WB(3)
IHC(1)
IF(1)
FC(1)
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Also for DNAJB1 (NM_006145)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human DNAJB1 (NP_006136) produced in HEK293T cell.
Clone NameClone OTI1F9 IsotypeIgG2b
Species ReactivityHuman , Dog Concentration1.6 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 37.9 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens DnaJ heat shock protein family (Hsp40) member B1 (DNAJB1), transcript variant 1
Alternative NameHdj1; Hsp40; HSPF1; RSPH16B; Sis1
Database LinkNP_006136
Entrez Gene 3337 Human
Entrez Gene 476688 Dog
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJB1 (RC201762, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJB1.
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WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DNAJB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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WB Image
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and DNAJB1-Knockout 293T cells (KO, Cat# LC811758) were separated by SDS-PAGE and immunoblotted with anti-DNAJB1 monoclonal antibody TA502195,(1:100). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
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IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DNAJB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502195)
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IF Image
Anti-DNAJB1 mouse monoclonal antibody (TA502195) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNAJB1(RC201762).
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FC Image
HEK293T cells transfected with either RC201762 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DNAJB1 antibody(TA502195), and then analyzed by flow cytometry.
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