Anti-CLPP Antibody, clone 1D8

Also for CLPP (NM_006012)

Gene Name	Homo sapiens ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli) (CLPP), nuclear gene encoding mitochondrial protein
Synonyms:	ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli); ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog; ATP-dependent protease ClpAP, proteolytic subunit, human; endopeptidase Clp
Immunogen	Full length human recombinant protein of human CLPP (NP_006003) produced in HEK293T cell.
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 1D8	Isotype	IgG2a
Species Reactivity	Human, Dog, Rat, Monkey	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IF, FC	Suggested Dilutions	WB 1:500~2000, IF 1:100, FLOW 1:100
Background	The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLPP (RC200301, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLPP.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CLPP monoclonal antibody.

IF Image

Anti-CLPP mouse monoclonal antibody (TA502062) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CLPP(RC200301).

FC Image

HEK293T cells transfected with either RC200301 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CLPP antibody(TA502062), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Hela cells, using anti-CLPP antibody(TA502062),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-CLPP antibody(TA502062),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

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Anti-CLPP TrueMAB Antibody clone 1D8

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