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Home Antibody All anti-PRKD2 antibodies

Anti-PRKD2 TRUEMAB Antibody Clone OTI5G1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA501672
  • anti-PRKD2 (Protein Kinase D2) mouse monoclonal antibody, clone OTI5G1 (formerly 5G1)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402555) , 20ug Explanation
100ul $325 In Stock
CF501672
  • Carrier-free (BSA/glycerol-free) PRKD2 mouse monoclonal antibody, clone OTI5G1 (formerly 5G1)
100ug $450 3-4 weeks

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(2)
IHC(4)
IF(1)
FC(1)
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Also for PRKD2 (NM_016457)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human PRKD2 (NP_057541) produced in HEK293T cell.
Clone NameClone OTI5G1 IsotypeIgG2b
Species ReactivityHuman, Dog, Rat, Monkey Concentration0.72 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
Predicted MW Explanation 96.5 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens protein kinase D2 (PRKD2), transcript variant 1
Alternative NameHSPC187; nPKC-D2; PKD2
Database LinkNP_057541
Entrez Gene 25865 Human
Entrez Gene 292658 Rat
Entrez Gene 484427 Dog
FunctionThe protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].
Related PathwayProtein KinaseDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKD2 (RC215335, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKD2.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKD2 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PRKD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501672, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PRKD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501672, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PRKD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501672, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PRKD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501672, Dilution 1:50)
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Anti-PRKD2 mouse monoclonal antibody (TA501672) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKD2(RC215335).
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HEK293T cells transfected with either RC215335 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PRKD2 antibody(TA501672), and then analyzed by flow cytometry.
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