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Anti-PRKD2 TRUEMAB Antibody clone 5A3
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for PRKD2 (NM_016457)
|Full length human recombinant protein of human PRKD2 (NP_057541) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens protein kinase D2 (PRKD2), transcript variant 1|
|HSPC187; nPKC-D2; PKD2|
Entrez Gene 25865 Human
|The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKD2 (RC215335, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKD2.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PRKD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501648)
Anti-PRKD2 mouse monoclonal antibody (TA501648) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKD2(RC215335).
HEK293T cells transfected with either RC215335 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PRKD2 antibody(TA501648), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-PRKD2 antibody(TA501648),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-PRKD2 antibody(TA501648),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).