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Home Antibody All anti-UBE2T antibodies

Anti-UBE2T TRUEMAB Antibody Clone OTI2F5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA501645 anti-UBE2T mouse monoclonal antibody, clone OTI2F5 (formerly 2F5) 100ul $379 In Stock
LC402285 UBE2T HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501645 Carrier-free (BSA/glycerol-free) UBE2T mouse monoclonal antibody, clone OTI2F5 (formerly 2F5) 100ug $450 3-4 weeks
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WB(2)
IF(1)
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Also for UBE2T (NM_014176)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human UBE2T (NP_054895) produced in HEK293T cell.
Clone NameClone OTI2F5 IsotypeIgG2a
Species ReactivityHuman Concentration0.33 mg/ml
Guaranteed Application *WB, IF Suggested DilutionsWB 1:500, IF 1:100,
Predicted MW Explanation 22.3 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens ubiquitin-conjugating enzyme E2T (UBE2T)
Alternative NameHSPC150; PIG50
Database LinkNP_054895
Entrez Gene 29089 Human
FunctionThe covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]).[supplied by OMIM].
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2T (RC200748, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2T.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UBE2T monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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Anti-UBE2T mouse monoclonal antibody (TA501645) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2T(RC200748).
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