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Anti-PKLR TRUEMAB Antibody clone 1C7
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for PKLR (NM_000298)
|Full length human recombinant protein of human PKLR (NP_000289) produced in HEK293T cell.|
|WB, IF, FC
||WB 1:2000, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant 1|
|PK1; PKL; PKR; PKRL; RPK|
|The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKLR (RC206455, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKLR.
Anti-PKLR mouse monoclonal antibody (TA501472) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PKLR(RC206455).
Flow cytometric Analysis of Jurkat cells, using anti-PKLR antibody(TA501472),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).