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Anti-PKLR TrueMAB Antibody clone 1H2
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for PKLR (NM_000298)
|Full length human recombinant protein of human PKLR (NP_000289) produced in HEK293T cell.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
||WB 1:2000, IF 1:100, FLOW 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens pyruvate kinase, liver and RBC (PKLR), nuclear gene encoding mitochondrial protein, transcript variant 1|
|PK1; PKL; PKR; PKRL; RPK|
|The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKLR (RC206455, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKLR.
Anti-PKLR mouse monoclonal antibody (TA501416) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PKLR(RC206455).
HEK293T cells transfected with either RC206455 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PKLR antibody(TA501416), and then analyzed by flow cytometry.