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Home Antibody All anti-HIBCH antibodies

Anti-HIBCH TRUEMAB Antibody Clone OTI3D11

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA501387 anti-HIBCH mouse monoclonal antibody, clone OTI3D11 (formerly 3D11) 100ul $325 In Stock
LC402324 HIBCH HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501387 Carrier-free (BSA/glycerol-free) HIBCH mouse monoclonal antibody, clone OTI3D11 (formerly 3D11) 100ug $450 3-4 weeks
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WB(2)
IF(1)
FC(1)
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Also for HIBCH (NM_014362)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human HIBCH (NP_055177) produced in HEK293T cell.
Clone NameClone OTI3D11 IsotypeIgG1
Species ReactivityHuman, Dog, Rat, Monkey Concentration0.24 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:500~2000, IF 1:100, FLOW 1:100
Predicted MW Explanation 39.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant 1
Alternative NameHIBYLCOAH
Database LinkNP_055177
Entrez Gene 26275 Human
Entrez Gene 301384 Rat
Entrez Gene 607040 Dog
FunctionThis gene encodes the enzyme responsible for hydrolysis of both HIBYL-CoA and beta-hydroxypropionyl-CoA. Mutations in this gene have been associated with 3-hyroxyisobutyryl-CoA hydrolase deficiency. Alternative splicing results in multiple transcript variants.
Related Pathway Valine, leucine and isoleucine degradationbeta-Alanine metabolismPropanoate metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIBCH (RC209814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIBCH.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HIBCH monoclonal antibody.
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Anti-HIBCH mouse monoclonal antibody (TA501387) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HIBCH(RC209814).
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HEK293T cells transfected with either RC209814 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HIBCH antibody(TA501387), and then analyzed by flow cytometry.
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