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Home Antibody All anti-BDH2 antibodies

Anti-BDH2 TRUEMAB Antibody Clone OTI4G4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA501291 anti-BDH2 mouse monoclonal antibody, clone OTI4G4 (formerly 4G4) 100ul $325 In Stock
LC402755 BDH2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501291 Carrier-free (BSA/glycerol-free) BDH2 mouse monoclonal antibody, clone OTI4G4 (formerly 4G4) 100ug $450 3-4 weeks
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WB(2)
IHC(1)
IF(1)
FC(3)

OriGene Data

ImmunogenFull length human recombinant protein of human BDH2(NP_064524) produced in HEK293T cell.
Clone NameClone OTI4G4 IsotypeIgG2a
Species ReactivityHuman Concentration0.73 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100
Predicted MW Explanation 26.5 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens 3-hydroxybutyrate dehydrogenase, type 2 (BDH2)
Alternative NameDHRS6; EFA6R; PRO20933; SDR15C1; UCPA-OR; UNQ6308
Database LinkNP_064524
Entrez Gene 56898 Human
Function
Related PathwayDruggable Genome Synthesis and degradation of ketone bodiesButanoate metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BDH2 (RC210586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BDH2.
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Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-BDH2 monoclonal antibody.(1:200)
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501291, Dilution 1:50)
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Anti-BDH2 mouse monoclonal antibody (TA501291) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BDH2(RC210586).
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HEK293T cells transfected with either RC210586 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BDH2 antibody(TA501291), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Hela cells, using anti-BDH2 antibody(TA501291),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-BDH2 antibody(TA501291),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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