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Home Antibody All anti-H6PD antibodies

Anti-H6PD TRUEMAB Antibody Clone OTI2A7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA501257 anti-H6PD mouse monoclonal antibody, clone OTI2A7 (formerly 2A7) 100ul
30ul
$379 In Stock
LC401369 H6PD HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501257 Carrier-free (BSA/glycerol-free) H6PD mouse monoclonal antibody, clone OTI2A7 (formerly 2A7) 100ug $450 3-4 weeks
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WB(3)
IHC(4)
IF(1)
FC(2)

OriGene Data

ImmunogenFull length human recombinant protein of human H6PD (NP_004276) produced in HEK293T cell.
Clone NameClone OTI2A7 IsotypeIgG1
Species ReactivityHuman, Monkey Concentration0.52 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:250~500, IHC 1:50, IF 1:100, FLOW 1:100
Predicted MW Explanation 88.7 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) (H6PD), transcript variant 2
Alternative NameCORTRD1; G6PDH; GDH
Database LinkNP_004276
Entrez Gene 9563 Human
Entrez Gene 710107 Monkey
FunctionThere are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells.
Related Pathway Pentose phosphate pathwayMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY H6PD (RC209890, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-H6PD .
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-H6PD monoclonal antibody.
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-H6PD monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-H6PD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501257, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-H6PD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501257, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-H6PD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501257, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-H6PD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501257, Dilution 1:50)
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Anti-H6PD mouse monoclonal antibody (TA501257) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY H6PD (RC209890).
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HEK293T cells transfected with either RC209890 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-H6PD antibody(TA501257), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Jurkat cells, using anti-H6PD antibody(TA501257),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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