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Anti-BIRC5 TRUEMAB Antibody Clone OTI2D9
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for BIRC5 (NM_001168)
|Full length human recombinant protein of human BIRC5 (NP_001159) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:2000, IF 1:100, FLOW 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens baculoviral IAP repeat containing 5 (BIRC5), transcript variant 1|
Entrez Gene 332 Human
|This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined.|
|Stem cell - PluripotencyDruggable Genome Pathways in cancerColorectal cancer|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIRC5 (RC205935, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC5 .
Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-BIRC5 monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BIRC5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA501245, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BIRC5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA501245, Dilution 1:50)
Anti-BIRC5 mouse monoclonal antibody (TA501245) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BIRC5 (RC205935).
HEK293T cells transfected with either RC205935 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BIRC5 antibody(TA501245), and then analyzed by flow cytometry.