Anti-BIRC5 Antibody, clone 2D9

Also for BIRC5 (NM_001168)

OriGene Data

Immunogen	Full length human recombinant protein of human BIRC5 (NP_001159) produced in HEK293T cell.
Clone Name	clone 2D9	Isotype	IgG1
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IF, FC	Suggested Dilutions	WB 1:2000, IF 1:100, FLOW 1:100
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens baculoviral IAP repeat containing 5 (BIRC5), transcript variant 1
Alternative Name	API4; EPR-1
Database Link	NP_001159
Function	This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined.
Related Pathway	Apoptosis Cell cycle

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIRC5 (RC205935, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC5 .

IF Image

Anti-BIRC5 mouse monoclonal antibody (TA501245) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BIRC5 (RC205935).

FC Image

HEK293T cells transfected with either RC205935 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BIRC5 antibody(TA501245), and then analyzed by flow cytometry.

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Anti-BIRC5 TrueMAB Antibody clone 2D9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data