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Home Antibody All anti-PFN1 antibodies

Anti-PFN1 TRUEMAB Antibody Clone OTI2D2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA501243 anti-PFN1 (Profilin 1) mouse monoclonal antibody, clone OTI2D2 (formerly 2D2) 100ul $379 In Stock
LC417584 PFN1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501243 Carrier-free (BSA/glycerol-free) PFN1 mouse monoclonal antibody, clone OTI2D2 (formerly 2D2) 100ug $450 3-4 weeks
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WB(2)
IHC(3)
IF(1)
FC(3)

OriGene Data

ImmunogenFull length human recombinant protein of human PFN1 (NP_005013) produced in HEK293T cell.
Clone NameClone OTI2D2 IsotypeIgG1
Species ReactivityHuman, Dog, Rat, Monkey Concentration0.58 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
Predicted MW Explanation 14.9 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens profilin 1 (PFN1)
Alternative NameALS18
Database LinkNP_005013
Entrez Gene 5216 Human
Entrez Gene 64303 Rat
Entrez Gene 710753 Monkey
Entrez Gene 607397 Dog
FunctionThe protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome. [provided by RefSeq].
Related PathwayStem cell - PluripotencyDruggable Genome Regulation of actin cytoskeleton

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFN1 (RC202338, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PFN1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PFN1 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PFN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501243, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PFN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501243, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PFN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501243, Dilution 1:50)
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Anti-PFN1 mouse monoclonal antibody (TA501243) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PFN1(RC202338).
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HEK293T cells transfected with either RC202338 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PFN1 antibody(TA501243), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Hela cells, using anti-PFN1 antibody(TA501243),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-PFN1 antibody(TA501243),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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