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Anti-PFN1 TRUEMAB Antibody clone 1D5
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for PFN1 (NM_005022)
|Full length human recombinant protein of human PFN1 (NP_005013) produced in HEK293T cell.|
|Human, Dog, Rat, Monkey
|WB, IHC, IF, FC
||WB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens profilin 1 (PFN1)|
|The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFN1 (RC202338, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PFN1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PFN1 monoclonal antibody.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-PFN1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PFN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501211, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PFN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501211, Dilution 1:50)
Anti-PFN1 mouse monoclonal antibody (TA501211) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PFN1(RC202338 ).
HEK293T cells transfected with either RC202338 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PFN1 antibody(TA501211), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-PFN1 antibody(TA501211),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-PFN1 antibody(TA501211),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).