Anti-SHC1 Antibody, clone 3H1

Also for SHC1 (NM_003029)

OriGene Data

Immunogen	Full length human recombinant protein of human SHC1 (NP_003020) produced in HEK293T cell.
Clone Name	clone 3H1	Isotype	IgG1
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IF, FC	Suggested Dilutions	WB 1:2000, IF 1:100, Flow 1:100
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens SHC (Src homology 2 domain containing) transforming protein 1 (SHC1), transcript variant 2
Alternative Name	SHC; SHCA
Database Link	NP_003020
Function	Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span
Related Pathway	EGFR1 Signaling Pathway Focal Adhesion Hemostasis

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHC1 (RC204362, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHC1.

IF Image

Anti-SHC1 mouse monoclonal antibody (TA501103) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SHC1(RC204362).

FC Image

HEK293T cells transfected with either pCMV6-ENTRY SHC1(RC204362)(Red) or empty vector control plasmid(Blue) were immunostained with anti-SHC1 mouse monoclonal(TA501103), and then analyzed by flow cytometry.

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Anti-SHC1 TrueMAB Antibody clone 3H1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data