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Anti-NIT1 TRUEMAB Antibody clone 3A11
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for NIT1 (NM_005600)
|Full length human recombinant protein of human NIT1 (NP_005591) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens nitrilase 1 (NIT1), transcript variant 1|
|Play a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. it is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2.|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT1 (RC211519, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT1.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-NIT1 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-NIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501084, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-NIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501084, Dilution 1:50)
Anti-NIT1 mouse monoclonal antibody (TA501084) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT1(RC211519).
Immunofluorescent staining of HT29 cells using anti-NIT1 mouse monoclonal antibody (TA501084).
HEK293T cells transfected with either pCMV6-ENTRY NIT1(RC211519)(Red) or empty vector control plasmid(Blue) were immunostained with anti-NIT1 mouse monoclonal(TA501084), and then analyzed by flow cytometry.