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Anti-RIC8A TRUEMAB Antibody Clone 3G3
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for RIC8A (NM_021932)
|Full length human recombinant protein of human RIC8A (NP_068751) produced in HEK293T cell.|
|WB, IHC, IF
||WB 1:1000, IHC 1:50, IF 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens RIC8 guanine nucleotide exchange factor A (RIC8A), transcript variant 1|
Entrez Gene 60626 Human
|Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIC8A (RC211359, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIC8A.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-RIC8A monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-RIC8A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501055, Dilution 1:50)
Anti-RIC8A mouse monoclonal antibody (TA501055) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RIC8A(RC211359).
Immunofluorescent staining of HepG2 cells using anti-RIC8A mouse monoclonal antibody (TA501055).