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Anti-RIC8A TRUEMAB Antibody Clone OTI3G3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations (1) Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA501055 anti-RIC8A (RIC8) mouse monoclonal antibody, clone OTI3G3 (formerly 3G3) 100ul 379
In Stock
LC402886 RIC8A HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501055 Carrier-free (BSA/glycerol-free) RIC8A mouse monoclonal antibody, clone OTI3G3 (formerly 3G3) 100ug $450 3-4 Days
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Also for RIC8A (NM_021932)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human RIC8A (NP_068751) produced in HEK293T cell.
Clone NameClone OTI3G3 IsotypeIgG1
Species ReactivityHuman Concentration0.83 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:1000, IHC 1:50, IF 1:100,
Predicted MW Explanation 59.6 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens RIC8 guanine nucleotide exchange factor A (RIC8A), transcript variant 1
Alternative NameRIC8
Database LinkNP_068751
Entrez Gene 60626 Human
FunctionGuanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIC8A (RC211359, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIC8A.

WB Image
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-RIC8A monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

IHC Image
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-RIC8A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501055, Dilution 1:50)

IF Image
Anti-RIC8A mouse monoclonal antibody (TA501055) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RIC8A(RC211359).

IF Image
Immunofluorescent staining of HepG2 cells using anti-RIC8A mouse monoclonal antibody (TA501055).


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