Anti-MCL1 Antibody, Clone 10D10

Also for MCL1 (NM_021960)

Gene Name	Homo sapiens myeloid cell leukemia sequence 1 (BCL2-related) (MCL1), nuclear gene encoding mitochondrial protein, transcript variant 1
Synonyms:	bcl2-L-3; BCL2L3; EAT; Mcl-1; MCL1-ES; mcl1/EAT; MCL1L; MCL1S; TM
Immunogen	Full length human recombinant protein of human MCL1 (NP_068779) produced in HEK293T cell.
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	Clone 10D10	Isotype	IgG2a
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
Background	The protein encoded by this gene belongs to the Bcl-2 family. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.
Related Pathway	Apoptosis

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (RC200521, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1.

IHC Image

Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-MCL1 mouse monoclonal antibody. (TA500990, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-MCL1 mouse monoclonal antibody. (TA500990, Dilution 1:50)

IF Image

Anti-MCL1 mouse monoclonal antibody (TA500990) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCL1(RC200521).

FC Image

HEK293T cells transfected with either pCMV6-ENTRY MCL1(RC200521)(Red) or empty vector control plasmid(Blue) were immunostained with anti-MCL1 mouse monoclonal(TA500990), and then analyzed by flow cytometry.

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Anti-MCL1 TrueMAB Antibody Clone 10D10

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