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Anti-MCL1 TRUEMAB Antibody Clone OTI10D10
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for MCL1 (NM_021960)
|Full length human recombinant protein of human MCL1 (NP_068779) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens myeloid cell leukemia 1 (MCL1), transcript variant 1|
|bcl2-L-3; BCL2L3; EAT; Mcl-1; MCL1-ES; mcl1/EAT; MCL1L; MCL1S; TM|
Entrez Gene 4170 Human
|The protein encoded by this gene belongs to the Bcl-2 family. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.|
|TransmembraneDruggable Genome |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (RC200521, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1.
Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-MCL1 monoclonal antibody at 1:200 dilution.
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500990, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500990, Dilution 1:50)
Anti-MCL1 mouse monoclonal antibody (TA500990) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCL1(RC200521).
HEK293T cells transfected with either pCMV6-ENTRY MCL1(RC200521)(Red) or empty vector control plasmid(Blue) were immunostained with anti-MCL1 mouse monoclonal(TA500990), and then analyzed by flow cytometry.