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Home Antibody All anti-RALBP1 antibodies

Anti-RALBP1 TRUEMAB Antibody Clone OTI6C6

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA500897 anti-RALBP1 (RIP1) mouse monoclonal antibody, clone OTI6C6 (formerly 6C6) 100ul $325 In Stock
LC402031 RALBP1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500897 Carrier-free (BSA/glycerol-free) RALBP1 mouse monoclonal antibody, clone OTI6C6 (formerly 6C6) 100ug $450 4 Weeks
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Also for RALBP1 (NM_006788)
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OriGene Data

ImmunogenFull length human recombinant protein of human RALBP1 (NP_006779) produced in HEK293T cell.
Clone NameClone OTI6C6 IsotypeIgG1
Species ReactivityHuman Concentration0.68 mg/ml
Guaranteed Application *WB, IF, IP, FC Suggested DilutionsWB 1:2000, IF 1:100, Flow 1:100, IP: 4ug/mL
Predicted MW Explanation 76.0 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameralA binding protein 1
Alternative NameRIP1|RLIP1|RLIP76
Database LinkNP_006779
Entrez Gene 10928 Human
FunctionCan activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDC2 to switch off endocytosis, One of its substrates would be EPN1/Epsin
Related Pathway Pathways in cancerPancreatic cancer

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RALBP1 (RC201524, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RALBP1.

WB Image
Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-RALBP1 monoclonal antibody.(1:200)

IF Image
Anti-RALBP1 mouse monoclonal antibody (TA500897) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RALBP1(RC201524).

IP Image
Immunoprecipitation(IP) of RALBP1 by using TrueMab monoclonal anti-RALBP1 antibodies (Negative control: IP without adding anti-RALBP1 antibody.). For each experiment, 500ul of DDK tagged RALBP1 overexpression lysates (at 1:5 dilution with HEK293T lysate),2ug of anti-RALBP1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

FC Image
HEK293T cells transfected with either pCMV6-ENTRY RALBP1(RC201524)(Red) or empty vector control plasmid(Blue) were immunostained with anti-RALBP1 mouse monoclonal(TA500897), and then analyzed by flow cytometry.

FC Image
Flow cytometric analysis of Hela cells, using anti-RALBP1 antibody(TA500897),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).


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