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ACO2 (Gene ID 50) Antibody

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Catalog No. Description Amount Price Shipping *
TA500873
  • Purified anti-ACO2 mouse monoclonal antibody, clone 7G4
100 ul $379 Next Day
  • HEK293T cell transient overexpression lysate (LY400442) as a positive control.
20 ug Included for FREE
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WB(1)
IF(2)
FC(1)
 
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Also for ACO2 (NM_001098)
Gene NameHomo sapiens aconitase 2, mitochondrial (ACO2), nuclear gene encoding mitochondrial protein
Synonyms:ACONM; MGC20605; MGC33908
ImmunogenFull length human recombinant protein of human ACO2 (NP_001089) produced in HEK293T cell.
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone NameClone 7G4 IsotypeIgG1
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:2000, IF 1:100, Flow 1:100
BackgroundThe protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.
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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACO2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACO2.
IF Image
Anti-ACO2 mouse monoclonal antibody (TA500873) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACO2(RC204307).
IF Image
Immunofluorescent staining of HepG2 cells using anti-ACO2 mouse monoclonal antibody (TA500873).
FC Image
HEK293T cells transfected with either pCMV6-ENTRY ACO2(RC204307)(Red) or empty vector control plasmid(Blue) were immunostained with anti-ACO2 mouse monoclonal(TA500873), and then analyzed by flow cytometry.

 

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