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Home Antibody All anti-HK2 antibodies

Anti-HK2 TRUEMAB Antibody Clone OTI4C5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500856 anti-HK2 (Hexokinase II) mouse monoclonal antibody, clone OTI4C5 (formerly 4C5) 100ul $379 In Stock
LC400068 HK2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500856 Carrier-free (BSA/glycerol-free) HK2 mouse monoclonal antibody, clone OTI4C5 (formerly 4C5) 100ug $450 3-4 weeks
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WB(2)
IF(2)
FC(1)

OriGene Data

ImmunogenFull length human recombinant protein of human HK2 (NP_000180) produced in HEK293T cell.
Clone NameClone OTI4C5 IsotypeIgG1
Species ReactivityHuman, Dog Concentration0.87 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:1000, IF 1:100, Flow 1:100
Predicted MW Explanation 102.4 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens hexokinase 2 (HK2)
Alternative NameHKII; HXK2
Database LinkNP_000180
Entrez Gene 3099 Human
Entrez Gene 100856448 Dog
FunctionHexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways.This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
Related PathwayDruggable Genome Glycolysis / GluconeogenesisFructose and mannose metabolismGalactose metabolismStarch and sucrose metabolismAmino sugar and nucleotide sugar metabolismMetabolic pathwaysMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HK2 (RC209482, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HK2.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HK2 monoclonal antibody.
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Anti-HK2 mouse monoclonal antibody (TA500856) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HK2(RC209482).
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Immunofluorescent staining of HepG2 cells using anti-HK2 mouse monoclonal antibody (TA500856).
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HEK293T cells transfected with either pCMV6-ENTRY HK2(RC209482)(Red) or empty vector control plasmid(Blue) were immunostained with anti-HK2 mouse monoclonal(TA500856), and then analyzed by flow cytometry.
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