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 Home All anti-ATP5B antibodies

Anti-ATP5B TrueMAB Antibody clone 4E5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Related Products Conjugation/Bulk FAQs
SKU Description Amount Price Availability*  
TA500833
  • Purified anti-ATP5B mouse monoclonal antibody, clone 4E5
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400637) , 20ug
 100ul $325 In Stock Add to Shopping Cart
CF500833
  • Carrier-free (BSA/glycerol-free) ATP5B mouse monoclonal antibody, clone 4E5
100ug $450 3-4 weeks Add to Shopping Cart
WB(2)
IHC(11)
IF(1)
FC(2)
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Also for ATP5B (NM_001686)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
Gene NameHomo sapiens ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide (ATP5B), nuclear gene encoding mitochondrial protein
Synonyms:ATPMB; ATPSB
ImmunogenFull length human recombinant protein of human ATP5B (NP_001677) produced in HEK293T cell.
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Nameclone 4E5 IsotypeIgG2a
Species ReactivityHuman, Dog, Rat, Monkey Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:1000, IHC 1:50, IF 1:100, Flow 1:100
BackgroundThis gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis,utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (RC201638, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATP5B monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded breast tissue using anti-ATP5B mouse monoclonal antibody. (TA500833, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ATP5Bmouse monoclonal antibody. (TA500833, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded liver tissue using anti-ATP5Bmouse monoclonal antibody. (TA500833, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded lung tissue using anti-ATP5Bmouse monoclonal antibody. (TA500833, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-ATP5Bmouse monoclonal antibody. (TA500833, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ATP5Bmouse monoclonal antibody. (TA500833, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-ATP5Bmouse monoclonal antibody. (TA500833, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded thyroid tissue using anti-ATP5Bmouse monoclonal antibody. (TA500833, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-ATP5Bmouse monoclonal antibody. (TA500833, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ATP5Bmouse monoclonal antibody. (TA500833, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-ATP5Bmouse monoclonal antibody. (TA500833, Dilution 1:50)
IF Image
Anti-ATP5B mouse monoclonal antibody (TA500833) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP5B(RC201638).
FC Image
Flow cytometric analysis of Hela cells, using anti-ATP5B antibody(TA500833),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
FC Image
Flow cytometric analysis of Jurkat cells, using anti-ATP5B antibody(TA500833),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).

 

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