Anti-HSPA1A Antibody, clone 1B2

Also for HSPA1A (NM_005345)

OriGene Data

Immunogen	Full length human recombinant protein of human HSPA1A (NP_005336) produced in HEK293T cell.
Clone Name	clone 1B2	Isotype	IgG1
Species Reactivity	Human, Dog, Monkey	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC	Suggested Dilutions	WB 1:2000, IHC 1:50,
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens heat shock 70kDa protein 1A (HSPA1A)
Alternative Name	HSP70-1; HSP70-1A; HSP70I; HSP72; HSPA1
Database Link	NP_005336
Function	This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
Related Pathway	MAPK signaling pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA1A (RC200270, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA1A.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPA1A monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-HSPA1Amouse monoclonal antibody. (TA500827, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded liver tissue using anti-HSPA1Amouse monoclonal antibody. (TA500827, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-HSPA1Amouse monoclonal antibody. (TA500827, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-HSPA1Amouse monoclonal antibody. (TA500827, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-HSPA1Amouse monoclonal antibody. (TA500827, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded prostate tissue using anti-HSPA1Amouse monoclonal antibody. (TA500827, Dilution 1:50)

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Anti-HSPA1A TrueMAB Antibody clone 1B2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data