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Home Antibody All anti-AKT2 antibodies

Anti-AKT2 TRUEMAB Antibody Clone OTI4H7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500814 AKT2 mouse monoclonal antibody, clone OTI4H7 (formerly 4H7) 100ul
30ul
$379 In Stock
LC419836 AKT2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500814 Carrier-free (BSA/glycerol-free) AKT2 mouse monoclonal antibody, clone OTI4H7 (formerly 4H7) 100ug $450 3-4 weeks
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WB(2)
IHC(2)
IF(2)
FC(2)

OriGene Data

ImmunogenFull length human recombinant protein of human AKT2 (NP_001617) produced in HEK293T cell.
Clone NameClone OTI4H7 IsotypeIgG1
Species ReactivityHuman Concentration0.56 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:1000, IHC 1:50, IF 1:50~100, FLOW 1:100,
Predicted MW Explanation 55.6 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens v-akt murine thymoma viral oncogene homolog 2 (AKT2), transcript variant 1
Alternative NameHIHGHH; PKBB; PKBBETA; PRKBB; RAC-BETA
Database LinkNP_001617
Entrez Gene 208 Human
FunctionThis gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq].
Related PathwayProtein KinaseES Cell Differentiation/IPSDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayApoptosisVEGF signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT2 (RC217733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT2.
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Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-AKT2 monoclonal antibodyat 1:200 dilution.
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AKT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500814)
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AKT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500814)
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Anti-AKT2 mouse monoclonal antibody (TA500814) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT2(RC217733).
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Immunofluorescent staining of HepG2 cells using anti-AKT2 mouse monoclonal antibody (TA500814).
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Flow cytometric Analysis of Hela cells, using anti-AKT2 antibody(TA500814),(Red), compared to a nonspecific negative control antibody,(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-AKT2 antibody(TA500814),(Red), compared to a nonspecific negative control antibody,(Blue).
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