OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-RPA2 antibodies

Anti-RPA2 TRUEMAB Antibody Clone OTI8B3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

div

Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500788 RPA2 mouse monoclonal antibody, clone OTI8B3 (formerly 8B3) 100ul $325 In Stock
LC401031 RPA2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500788 Carrier-free (BSA/glycerol-free) RPA2 mouse monoclonal antibody, clone OTI8B3 (formerly 8B3) 100ug $450 3-4 weeks
Add to Shopping Cart

spacer
WB(1)
IP(1)
IHC(9)
IF(2)
FC(2)

OriGene Data

ImmunogenFull length human recombinant protein of human RPA2 (NP_002937) produced in HEK293T cell.
Clone NameClone OTI8B3 IsotypeIgG1
Species ReactivityHuman Concentration0.6 mg/ml
Guaranteed Application *WB, IHC, IF, IP, FC Suggested DilutionsWB 1:4000, IHC 1:50, IF 1:50~100, FLOW 1:100, IP 2ug/500ul
Predicted MW Explanation 29.1 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens replication protein A2, 32kDa (RPA2), transcript variant 1
Alternative NameREPA2; RP-A p32; RP-A p34; RPA32
Database LinkNP_002937
Entrez Gene 6118 Human
Function
Related PathwayStem cell - PluripotencyDruggable Genome DNA replicationNucleotide excision repairMismatch repairHomologous recombination

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.
spacer
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
spacer
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
spacer
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
spacer
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
spacer
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
spacer
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
spacer
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
spacer
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
spacer
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
spacer
Anti-RPA2 mouse monoclonal antibody (TA500788) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2(RC205715).
spacer
Immunofluorescent staining of HepG2 cells using anti-RPA2 mouse monoclonal antibody (TA500788).
spacer
Immunoprecipitation(IP) of RPA2 by using TrueMab monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
spacer
Flow cytometric Analysis of Hela cells, using anti-RPA2 antibody(TA500788),(Red), compared to a nonspecific negative control antibody,(Blue).
spacer
Flow cytometric Analysis of Jurkat cells, using anti-RPA2 antibody(TA500788),(Red), compared to a nonspecific negative control antibody,(Blue).
spacer

 

spacer
Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title