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Anti-HDAC10 TRUEMAB Antibody Clone 5E2
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for HDAC10 (NM_032019)
|Full length human recombinant protein of human HDAC10 (NP_114408) produced in HEK293T cell.|
|WB, IHC, IP, FC
||WB 1:2000, IHC 1:50, Flow 1:100, IP 2-4ug/mg
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens histone deacetylase 10 (HDAC10), transcript variant 1|
Entrez Gene 83933 Human
|Acetylation of histone core particles modulates chromatin structure and gene expression. The opposing enzymatic activities of histone acetyltransferases and histone deacetylases, such as HDAC10, determine the acetylation status of histone tails|
|Transcription FactorsDruggable Genome |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC10 (RC218536, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC10.
Immunohistochemical staining of paraffin-embedded liver tissue using anti-HDAC10mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500787, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-HDAC10mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500787, Dilution 1:50)
Immunoprecipitation of HDAC10 by using TrueMab monoclonal anti-HDAC10 antibody (Negative control: IP without adding anti-HDAC10 antibody). For each experiment, 500ul of DDK tagged HDAC10 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of
Flow cytometric analysis of Hela cells, using anti-HDAC10 antibody(TA500787),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Flow cytometric analysis of Jurkat cells, using anti-HDAC10 antibody(TA500787),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).