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Anti-AKT2 TRUEMAB Antibody Clone OTI1A2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA500782 AKT2 mouse monoclonal antibody, clone OTI1A2 (formerly 1A2) 100ul 325
In Stock
LC419836 AKT2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500782 Carrier-free (BSA/glycerol-free) AKT2 mouse monoclonal antibody, clone OTI1A2 (formerly 1A2) 100ug $450 3-4 weeks
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OriGene Data

ImmunogenFull length human recombinant protein of human AKT2 (NP_001617) produced in HEK293T cell.
Clone NameClone OTI1A2 IsotypeIgG2a
Species ReactivityHuman Concentration0.65 mg/ml
Guaranteed Application *WB, FC Suggested DilutionsWB 1:1000, FLOW 1:100,
Predicted MW Explanation 55.6 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens v-akt murine thymoma viral oncogene homolog 2 (AKT2), transcript variant 1
Database LinkNP_001617
Entrez Gene 208 Human
FunctionThis gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq].
Related PathwayProtein KinaseES Cell Differentiation/IPSDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayApoptosisVEGF signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT2 (RC217733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AKT2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Flow cytometric Analysis of Hela cells, using anti-AKT2 antibody(TA500782),(Red), compared to a nonspecific negative control antibody,(Blue).


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